NOVEL ANTI-INFLAMMATORY AND IMMUNOMODULATION EFFECTS OF ROSE ON THE ENDOTHELIUM IN NORMAL AND HYPOXIC INVITRO CONDITIONS
DOI:
https://doi.org/10.48750/acv.221Keywords:
Inflammation, Molecular biology, RoseAbstract
Aims
Inflammation is common in clinical practice and often results in significant complications. The study was performed in search of a novel anti-inflammatory agent for inflammation modulation which would be useful for cardiovascular disorders and various clinical scenarios.
Materials and methods
A crushed red rose extract was prepared from the petals, and it was processed for analysis. The extract was tested on HUVEC cells at various concentrations. By microscopic analysis of cells, a safe concentration was identified, and the levels below the safe limit were tested at 72 hours and seven days for selected cytokines secretion.
Results
Majority of the tested Inflammatory cytokine secretion was reduced by the treatment of red rose extract on the cells. VEGF and angiogenic cytokine levels were reduced, but VEGF-R2 levels were maintained after the cell treatment. Below the safe concentration limit (0.5%) there were only minimal changes in the cytokines levels tested at various dilutions.
Conclusion
There is potential for a red rose extract treatment in the regulation of inflammatory cytokines secretion. Further studies need to be performed to identify the benefits.
Downloads
Download data is not yet available.
References
1. Aird W. The role of the endothelium in severe sepsis and multiple organ dysfunction syndrome. Blood. 2003;101(10):3765-3777.
2. Wang H, Ma S. The cytokine storm and factors determining the sequence and severity of organ dysfunction in multiple organ dysfunction syndrome. The American Journal of Emergency Medi- cine. 2008;26(6):711-715.
3. Ikei H, Komatsu M, Song C, Himoro E, Miyazaki Y. The physiological and psychological relaxing effects of viewing rose flowers in office workers. J Physiol Anthropol. 2014;33(1):6. Published 2014 Mar 8. doi:10.1186/1880-6805-33-6.
4. Komatsu M, Matsunaga K, Lee J, Ikei H, Song C, Himoro E, Miyazaki Y. The physiological and psychological relaxing effects of viewing rose flowers in medical staff. Jpn J Physiol Anthropol. 2013;18:1–7.
5. Song C, Igarashi M, Ikei H, Miyazaki Y. Physiological effects of viewing fresh red roses. Complementary Therapies in Medicine. 2017;35:78-84.
6. Boskabady MH, Shafei MN, Saberi Z, Amini S. Pharmacological effects of rosa damascena. Iran J Basic Med Sci. 2011;14(4):295-307.
7. Maleev A, Neshtev G, Stoianov S, Sheikov N. The ulcer protective and antiinflamatory effect of Bulgarian rose oil. Eksp Med Morfol. 1972;11:55–60.
8. Andoğan BC, Baydar H, Kaya S, Demirci M, Özbaşar D, Mumcu E. Anti- microbial activity and chemical composition of some essential oils. Arch Pharm Res. 2008;25:860–864.
9. Adwan G, Mhanna M. Synergistic effects of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimens. Middle East j sci res. 2008;3:134–139.
10. Hamidpour R, Hamidpour S, Hamidpour M, et al. Pelargonium grave- olens (Rose Geranium). A Novel Therapeutic Agent for Antibacterial, Antioxidant, Antifungal and Diabetics. Arch Can Res. 2017, 5:1. doi: 10.21767/2254-6081.1000134
11. Mahmood N, Piacente S, Pizza C, Burke A, Khan AL, Hay AJ. The anti-HIV activity and mechanisms of action of pure com-pounds isolated from Rosa damascena. Biochem Biophys Res Commun. 1996;229:73–79.
12. Gholamhoseinian A, Fallah H, sharifi-far F, Mirtajaddini M. The inhib- itory effect of some Iranian plantstracts on the alpha glucosidase. Iran J Basic Med Sci. 2008;11:1–9.
13. Boskabady MH, Vatanprast A, Parsee H, Ghasemzadeh M. Effect of aqueous-ethanolic extract from Rosa damascena on guinea pig isolated heart. Iran J Basic Med Sci. 2011;14:116–121.
14. Kwon EK, Lee DY, Lee H, Kim DO, Baek NI, Kim YE, et al. Flavo- noids from the Buds of Rosa damascena inhibit the Activity of 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase and Angio- tensin I-Converting Enzyme. J Agric Food Chem. 2010;58:882–886.
15. Jahromi HK, Jashni hk and Dialemeh S. Effect of Damask Rose Extract on FSH, LH and Testosterone Hormones in Rats. Int J Med Res Health Sci. 2016, 5, 5(S):267-271.
16. Biswas NR, Gupta SK, Das GK, Kumar N, Mongre PK, Haldar D, et al. Evaluation of ophthacare® eye drops - a herbal formulation in the management of various ophthalmic disorders. Phytother Res. 2001;15:618–620.
17. Gibbison B, López-López JA, Higgins JP, et al. Corticosteroids in septic shock: a systematic review and network meta-analysis. Crit Care. 2017;21(1):78. Published 2017 Mar 28. doi:10.1186/ s13054-017-1659-4.
18. Patel G, Balk R. Systemic Steroids in Severe Sepsis and Septic Shock. American Journal of Respiratory and Critical Care Medicine. 2012;185(2):133-139.
19. Venkatesh B, Finfer S, Cohen J, Rajbhandari D, Arabi Y, Bellomo R et al. Adjunctive Glucocorticoid Therapy in Patients with Septic Shock. New England Journal of Medicine. 2018;378(9):797-808.
20. Hörl W. Nonsteroidal Anti-Inflammatory Drugs and the Kidney. Phar- maceuticals. 2010;3(7):2291-2321.
21. Russell R. Non-steroidal anti-inflammatory drugs and gastroin- testinal damage---problems and solutions. Postgraduate Medical Journal. 2001;77(904):82-88.
22. Hoeben A. Vascular Endothelial Growth Factor and Angiogenesis. Pharmacological Reviews. 2004;56(4):549-580.
23. Shibuya M. Vascular Endothelial Growth Factor (VEGF) and Its Receptor (VEGFR) Signaling in Angiogenesis: A Crucial Target for Anti- and Pro-Angiogenic Therapies. Genes Cancer. 2011;2(12):1097-105.
24. Cébe-Suarez S, Zehnder-Fjällman A, Ballmer-Hofer K. The role of VEGF receptors in angiogenesis; complex partnerships. Cell Mol Life Sci. 2006;63(5):601-15.
25. Iikuni N, Lam QL, Lu L, Matarese G, La Cava A. Leptin and Inflamma- tion. Curr Immunol Rev. 2008;4(2):70-79.
26. Hakan Uzun, Ozan Bitik, Yahya Baltu, Çiğdem Sönmez, and Ayşegül Öztürk Kaymak, “The Effects of Reduction Mammaplasty on Serum Leptin Levels and Insulin Resistance,” International Journal of Endo- crinology, vol. 2015, Article ID 719824, 5 pages, 2015. https://doi. org/10.1155/2015/719824.
27. Grunnet LG, Aikin R, Tonnesen MF, et al. Proinflammatory cytokines activate the intrinsic apoptotic pathway in beta-cells. Diabetes. 2009;58(8):1807-15.
28. Sulkava M, Raitoharju E, Levula M, Seppälä I, Lyytikäinen L, Mennander A et al. Differentially expressed genes and canonical pathway expression in human atherosclerotic plaques – Tampere Vascular Study. Scientific Reports. 2017;7(1).
29. Porter JC, Hogg N (1999). Integrins take partners: cross-talk between integrins and other membrane receptors. Trends Cell Biol. 8 (10): 390–6. doi:10.1016/S0962-8924(98)01344-0. PMID 9789327.
30. Hubbard A, Rothlein R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radical Biology and Medicine. 2000;28(9):1379-1386.
31. Thundyil, J., Pavlovski, D., Sobey, C. G., and Arumugam, T. V. (2012). Adiponectin receptor signalling in the brain. Br. J. Pharmacol. 165, 313–327. doi: 10.1111/j.1476-5381.2011.01560.x
32. Lihn AS, Pedersen SB, Richelsen B. Adiponectin: action, regulation and association to insulin sensitivity. Obes Rev. 2005; 6(1):13-21.
33. Lo J, Ljubicic S, Leibiger B, Kern M, Leibiger I, Moede T et al. Adipsin Is an Adipokine that Improves
2. Wang H, Ma S. The cytokine storm and factors determining the sequence and severity of organ dysfunction in multiple organ dysfunction syndrome. The American Journal of Emergency Medi- cine. 2008;26(6):711-715.
3. Ikei H, Komatsu M, Song C, Himoro E, Miyazaki Y. The physiological and psychological relaxing effects of viewing rose flowers in office workers. J Physiol Anthropol. 2014;33(1):6. Published 2014 Mar 8. doi:10.1186/1880-6805-33-6.
4. Komatsu M, Matsunaga K, Lee J, Ikei H, Song C, Himoro E, Miyazaki Y. The physiological and psychological relaxing effects of viewing rose flowers in medical staff. Jpn J Physiol Anthropol. 2013;18:1–7.
5. Song C, Igarashi M, Ikei H, Miyazaki Y. Physiological effects of viewing fresh red roses. Complementary Therapies in Medicine. 2017;35:78-84.
6. Boskabady MH, Shafei MN, Saberi Z, Amini S. Pharmacological effects of rosa damascena. Iran J Basic Med Sci. 2011;14(4):295-307.
7. Maleev A, Neshtev G, Stoianov S, Sheikov N. The ulcer protective and antiinflamatory effect of Bulgarian rose oil. Eksp Med Morfol. 1972;11:55–60.
8. Andoğan BC, Baydar H, Kaya S, Demirci M, Özbaşar D, Mumcu E. Anti- microbial activity and chemical composition of some essential oils. Arch Pharm Res. 2008;25:860–864.
9. Adwan G, Mhanna M. Synergistic effects of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimens. Middle East j sci res. 2008;3:134–139.
10. Hamidpour R, Hamidpour S, Hamidpour M, et al. Pelargonium grave- olens (Rose Geranium). A Novel Therapeutic Agent for Antibacterial, Antioxidant, Antifungal and Diabetics. Arch Can Res. 2017, 5:1. doi: 10.21767/2254-6081.1000134
11. Mahmood N, Piacente S, Pizza C, Burke A, Khan AL, Hay AJ. The anti-HIV activity and mechanisms of action of pure com-pounds isolated from Rosa damascena. Biochem Biophys Res Commun. 1996;229:73–79.
12. Gholamhoseinian A, Fallah H, sharifi-far F, Mirtajaddini M. The inhib- itory effect of some Iranian plantstracts on the alpha glucosidase. Iran J Basic Med Sci. 2008;11:1–9.
13. Boskabady MH, Vatanprast A, Parsee H, Ghasemzadeh M. Effect of aqueous-ethanolic extract from Rosa damascena on guinea pig isolated heart. Iran J Basic Med Sci. 2011;14:116–121.
14. Kwon EK, Lee DY, Lee H, Kim DO, Baek NI, Kim YE, et al. Flavo- noids from the Buds of Rosa damascena inhibit the Activity of 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase and Angio- tensin I-Converting Enzyme. J Agric Food Chem. 2010;58:882–886.
15. Jahromi HK, Jashni hk and Dialemeh S. Effect of Damask Rose Extract on FSH, LH and Testosterone Hormones in Rats. Int J Med Res Health Sci. 2016, 5, 5(S):267-271.
16. Biswas NR, Gupta SK, Das GK, Kumar N, Mongre PK, Haldar D, et al. Evaluation of ophthacare® eye drops - a herbal formulation in the management of various ophthalmic disorders. Phytother Res. 2001;15:618–620.
17. Gibbison B, López-López JA, Higgins JP, et al. Corticosteroids in septic shock: a systematic review and network meta-analysis. Crit Care. 2017;21(1):78. Published 2017 Mar 28. doi:10.1186/ s13054-017-1659-4.
18. Patel G, Balk R. Systemic Steroids in Severe Sepsis and Septic Shock. American Journal of Respiratory and Critical Care Medicine. 2012;185(2):133-139.
19. Venkatesh B, Finfer S, Cohen J, Rajbhandari D, Arabi Y, Bellomo R et al. Adjunctive Glucocorticoid Therapy in Patients with Septic Shock. New England Journal of Medicine. 2018;378(9):797-808.
20. Hörl W. Nonsteroidal Anti-Inflammatory Drugs and the Kidney. Phar- maceuticals. 2010;3(7):2291-2321.
21. Russell R. Non-steroidal anti-inflammatory drugs and gastroin- testinal damage---problems and solutions. Postgraduate Medical Journal. 2001;77(904):82-88.
22. Hoeben A. Vascular Endothelial Growth Factor and Angiogenesis. Pharmacological Reviews. 2004;56(4):549-580.
23. Shibuya M. Vascular Endothelial Growth Factor (VEGF) and Its Receptor (VEGFR) Signaling in Angiogenesis: A Crucial Target for Anti- and Pro-Angiogenic Therapies. Genes Cancer. 2011;2(12):1097-105.
24. Cébe-Suarez S, Zehnder-Fjällman A, Ballmer-Hofer K. The role of VEGF receptors in angiogenesis; complex partnerships. Cell Mol Life Sci. 2006;63(5):601-15.
25. Iikuni N, Lam QL, Lu L, Matarese G, La Cava A. Leptin and Inflamma- tion. Curr Immunol Rev. 2008;4(2):70-79.
26. Hakan Uzun, Ozan Bitik, Yahya Baltu, Çiğdem Sönmez, and Ayşegül Öztürk Kaymak, “The Effects of Reduction Mammaplasty on Serum Leptin Levels and Insulin Resistance,” International Journal of Endo- crinology, vol. 2015, Article ID 719824, 5 pages, 2015. https://doi. org/10.1155/2015/719824.
27. Grunnet LG, Aikin R, Tonnesen MF, et al. Proinflammatory cytokines activate the intrinsic apoptotic pathway in beta-cells. Diabetes. 2009;58(8):1807-15.
28. Sulkava M, Raitoharju E, Levula M, Seppälä I, Lyytikäinen L, Mennander A et al. Differentially expressed genes and canonical pathway expression in human atherosclerotic plaques – Tampere Vascular Study. Scientific Reports. 2017;7(1).
29. Porter JC, Hogg N (1999). Integrins take partners: cross-talk between integrins and other membrane receptors. Trends Cell Biol. 8 (10): 390–6. doi:10.1016/S0962-8924(98)01344-0. PMID 9789327.
30. Hubbard A, Rothlein R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radical Biology and Medicine. 2000;28(9):1379-1386.
31. Thundyil, J., Pavlovski, D., Sobey, C. G., and Arumugam, T. V. (2012). Adiponectin receptor signalling in the brain. Br. J. Pharmacol. 165, 313–327. doi: 10.1111/j.1476-5381.2011.01560.x
32. Lihn AS, Pedersen SB, Richelsen B. Adiponectin: action, regulation and association to insulin sensitivity. Obes Rev. 2005; 6(1):13-21.
33. Lo J, Ljubicic S, Leibiger B, Kern M, Leibiger I, Moede T et al. Adipsin Is an Adipokine that Improves
Downloads
Published
2020-02-05
How to Cite
1.
Arokiaraj MC, Menesson E. NOVEL ANTI-INFLAMMATORY AND IMMUNOMODULATION EFFECTS OF ROSE ON THE ENDOTHELIUM IN NORMAL AND HYPOXIC INVITRO CONDITIONS. Angiol Cir Vasc [Internet]. 2020 Feb. 5 [cited 2024 Dec. 21];15(4):238-4. Available from: https://acvjournal.com/index.php/acv/article/view/221
Issue
Section
Original Article
